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Fluid Imaging Technologies FlowCAM User Manual

Fluid Imaging Technologies FlowCAM
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When an analysis is started, VisualSpreadsheet prompts the user for an experiment name to use for the
data files for this run. The program uses this name to prefix all of the data files for the run as well as to
use as the folder name where the data files will be saved.
VisualSpreadsheet stores the image data in non-compressed, lossless TIF file format with a *.tif file
extension. These files are named with the common prefix and a number appended. So if the common
prefix was 'my_experiment', then the image files would be named, my_experiment_000001.tif,
my_experiment_000002.tif, etc.
Run Files
A “.lst” (list) file is generated for each experimental analysis. List files are the primary data files for
FlowCAM experiments. List files are ASCII text files recording the fields of data collected for each
particle. The format of this file has changed several times but the VisualSpreadsheet program can
always read list files created by earlier versions of VisualSpreadsheet or older FlowCAM software. To
extract the data in a list file for use with other software including spreadsheet programs like MS Excel,
users can use the data Export functions in VisualSpreadsheet. All relevant data in a list file is made
available using the Export functions.
A context file with extension *.ctx is generated with each list file. It records the context information that
was in effect when the list file was generated or when the experiment was run. The name of the context
file uses the same common prefix as the other experiment files. The context file is a readable text file in
the common Windows 'ini' file format. The context dialog box, accessed through the Setup > Context
menu is used to modify the context settings.
The “notes” text file accompanies the context file. The name follows the same convention, common
prefix followed by _notes.txt. This file contains the experiment notes entered in the context dialog box.
Folders
User specific configuration and program behavior files are stored in a folder named VisualSpreadsheet
in the user’s My Documents folder. The folders and .ini file found in the VisualSpreadsheet folder
include:
· Class Templates: This is the folder where all class templates are stored by default. These
templates allows a user to pull in a set of classes to populate with a new data set.
· Context: This is where manually saved context files are stored.
· Default Context: This is where the context files that are “Saved as Local Default” are stored.
· Filters: Default location to save newly created filters.
· Layouts: Default location to save the Main window layout.
· Logs: Default location for logs, generated when selected by going to Help > Advanced Tools >
Advanced Settings > Logging.
· VisualSpreadsheet.ini file: Records and saves user preferences for window position, etc.

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Fluid Imaging Technologies FlowCAM Specifications

General IconGeneral
BrandFluid Imaging Technologies
ModelFlowCAM
CategoryMeasuring Instruments
LanguageEnglish

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