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The FlowCAM

is an integrated system for rapidly analyzing particles in a moving fluid. The

instrument combines selective capabilities of flow cytometry, microscopy, and fluorescence detection.

The FlowCAM automatically counts, images, and analyzes the particles or cells in a sample or a

continuous flow. Best of all, a FlowCAM can be customized to accommodate almost any environment

or application.

Originally developed for oceanographic investigations of organisms and particles in seawater, the

FlowCAM provides the user with the capability to rapidly evaluate particulate matter in fluids. The

instrument and software

provides the user with tools to quickly and efficiently meet challenges that

previously required multiple instruments and many hours of tedious work to complete. Your FlowCAM

has the following features and capabilities:

· High-Speed Digital Imaging

· Particle Size, Count, and Shape

· Real-Time Bulk and Individual Particle Analysis

· The Combined Benefits of Multiple Instruments

· Compact and Durable Packaging

· Ability to Image Particles 2 µm to 3 mm in Diameter

· Fluorescence Detection Providing Additional Selectivity

· Scatter Detection for Low Particle Concentrations

Basic Overview of the FlowCAM

In the FlowCAM system, sample is drawn into the flow chamber by a pump. Using the laser in Trigger

Mode, the photomultiplier tubes (PMTs) and scatter detector monitor the fluorescence and light scatter

of the passing particles. When a particle passing through the laser fan has sufficient fluorescence or

laser light scatter, the camera is triggered to take an image of the field of view. The fluorescence

value(s) and/or scatter detection value are then saved by VisualSpreadsheet (in addition to all other

particle properties). The computer, digital signal processor, and trigger circuitry work together to

initiate, retrieve and process images of the field of view. Groups of pixels that represent particles are

then “segmented” out of each raw image and saved as a separate collage image (along with all

associated parameter measurements). The process described above is similar when using AutoImage

Mode, except the camera is set to capture raw images at a user defined interval.

Fluid Imaging Technologies FlowCAM User Manual

Questions and Answers: