Pressure sensors
The most likely reason for pressure sensors to return incorrect values is incorrect
mounting:
•
Make sure that each flow cell is properly aligned with its adapter. Check that there
is no gap between flow cell and adapter. If necessary, loosen the screws and remount
the cell according to the instructions in Mount the main part tubing, on page 91. The
screws holding the latch pressing the flow cell against the adapter should be firmly
tightened (using finger force).
Flow meter
Problems with incorrect flow meter readings can be caused by:
SolutionProblem
Make sure Vaseline has been applied to the contact sur-
faces of the transducer connectors (see Mount the main
part tubing, on page 91).
Transducer surfaces not
treated with Vaseline.
Check that the pump tubing has been correctly mounted,
and that the pump lid is properly secured. Make sure the
pegs on the pump lid are turned all the way down (see
Connect and mount the pump tubing, on page 89).
Incorrectly mounted
pump tubing.
Make sure the TC clamps connecting the pump tubing to
the main part of the flow kit, and to the inlet tubing are
properly connected and that the TC gaskets are in right
position.
Poor pump tubing con-
nection.
Cavitation (small air
bubbles produced by
under-pressure), which
results in unstable flow
meter readings. Cavita-
tion is recognized by a
hissing noise.
118 ÄKTA ready Operating Instructions 28960345 AD
8 Component test
8.3 Troubleshooting a failed component test
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