pH calibration
pH calibration should be done at least once a day. Suitable pH buffer solutions are re-
quired.
It is recommended to use fresh pH buffer solutions at every calibration.
Note:
ActionStep
Two cups with the buffers representing the actual pH range are placed in
the small holder located on the inside of the front cabinet door.
1
Release the pH cable from its storage position by unscrewing its lower end
from the cabinet. Connect the pH electrode by screwing it onto the cable
(see Mount the main part tubing, on page 91 step pH electrode).
2
In the ÄKTA ready System Control module, on the System menu, click Cali-
brate.
3
Place the pH electrode in one of the buffers, enter the pH value for the buffer
in the Reference value 1 field, wait for the value to stabilize, and click Read
value 1.
4
Rinse the electrode in distilled water and place it in the other buffer solution.
Enter the pH value for the buffer in the Reference value 2 field, wait for the
value to stabilize, and click Read value 2.
5
Wait for response and, if electrode passed, click Close. Otherwise, click Close,
refresh/change the pH electrode, and repeat the calibration.
6
Sanitize the pH electrode by spraying it with 70% ethanol.7
Let the pH electrode dry and mount it as described in step 5 in Mount the
main part tubing, on page 91.
8
186 ÄKTA ready Operating Instructions 28960345 AD
12 Maintenance
12.1 Daily maintenance
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