1138.2 The test report ........................................................................................................................................
1178.3 Troubleshooting a failed component test .................................................................................
1229 Column installation .............................................................................................
1249.1 Columns ....................................................................................................................................................
1279.2 Installing a column ...............................................................................................................................
1359.3 Optional column test ...........................................................................................................................
1389.4 Optional column rinsing ....................................................................................................................
1399.5 Adding columns to the column list ...............................................................................................
14110 Creating a method ...............................................................................................
14210.1 The Method Editor ................................................................................................................................
14310.1.1 Introduction to the Method Editor ..........................................................................................
14510.1.2 Method Editor layout ....................................................................................................................
14710.1.3 Other Method Editor features ...................................................................................................
15210.2 An example of using the Method Editor .....................................................................................
15310.2.1 Creating the method and selecting a column ...................................................................
15410.2.2 Block 1: Opening valves ..............................................................................................................
15610.2.3 Block 2: Auto-zeroing pressure sensors ...............................................................................
15710.2.4 Block 3: Setting PID parameters ..............................................................................................
15810.2.5 Block 4: Filling the flow kit ..........................................................................................................
16010.2.6 Block 5: Equilibration ....................................................................................................................
16110.2.7 Block 6: Sample load ....................................................................................................................
16310.2.8 Block 7: Washing unbound sample .......................................................................................
16410.2.9 Block 8: Elution ................................................................................................................................
16610.2.10 Block 9: Regeneration ..................................................................................................................
16710.2.11 Saving the method ........................................................................................................................
16810.3 Creating a column efficiency test .................................................................................................
17011 Run a method ........................................................................................................
17111.1 Final preparations before a run .....................................................................................................
17511.2 Starting a run ..........................................................................................................................................
17811.3 Viewing and printing results ............................................................................................................
18011.4 After the run ............................................................................................................................................
18212 Maintenance .........................................................................................................
18412.1 Daily maintenance ...............................................................................................................................
18712.2 Yearly maintenance .............................................................................................................................
19412.3 Spare parts ..............................................................................................................................................
19512.4 Storage ......................................................................................................................................................
19613 Troubleshooting ...................................................................................................
20114 Reference information ........................................................................................
20214.1 Specifications .........................................................................................................................................
20614.2 Process wetted materials .................................................................................................................
20714.3 Chemical resistance ............................................................................................................................
21014.4 Ordering information ..........................................................................................................................
ÄKTA ready Operating Instructions 28960345 AD 3
Table of Contents