Integra MEDIAJET - Page 36

MEDIAJET – Operating instructions V09 Operation
36 INTEGRA Biosciences AG
4.5.2 Filling the tubing
 The preparations for the filling are now complete.
4.5.3 Calibration of the pump before filling
Before you start the actual filling process, you have the option of carrying out a
calibration of the dosing volume. This is particularly important if you work with
tubing(s) not obtained from INTEGRA Biosciences, as the dosing quantity is
directly dependent on the diameter of the tubing.
Biplate option
The double head pump squeezes two tubings in parallel. To guarantee a correct
filling volume, identical tubing must be used. Connect both tubing sets, calibrate
the pumps as described above with two graduated cylinders and enter the mean
of the measured volumes.
 Connect the tubing set(s) to the media
source.
 Keep the PRIME key pressed until the
tubing or both tubings (biplate option) are
completely filled respectively.
The empty Petri dish in the filling position of the
rotor prevents accidental contamination of the
filling chamber.
 Press CALIBRATE
 The dialogue window PUMP
CALIBRATION opens.
 Before you start the calibration, make sure
that the tubing is completely filled with
medium.
 Hold the filling nozzle in a suitable
graduated cylinder and press START or
DELAYED START (dosing will start after 5
seconds).
 Enter the volume measured and press
SAVE.
 Following calibration, insert the filling nozzle
into the filling nozzle holder.
 The pump is now calibrated.