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Olympus IX73 - Oil and Water Immersion Objectives

Olympus IX73
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31
IX73
4-7 Oil- or Water-Immersion Objective
a
} If you use an oil-immersion objective, use immersion oil as de-
scribed below.
Always use immersion oil supplied by Olympus.
Using a low-power objective, bring the specimen into focus.
Rotate the revolving nosepiece to engage the oil immersion objec-
tive.
Remove the specimen and move the stage insert cut-out
a
close
to the objective front lens. Apply a drop of the immersion oil to the
objective front lens. Place the specimen and rotate the fine adjust-
ment knob to bring the specimen into focus.
• Use as little oil as possible.
• If the oil contains air bubbles, the image will be degraded. Make
sure the oil is free of air bubbles.
After use, remove immersion oil from the objective front lens by wip-
ing with gauze slightly moistened with absolute alcohol.
} The presence of air bubbles can be checked by viewing the pupil
of the objective (viewed as a bright circular shape) in the tube after
removing the eyepiece and opening the field iris diaphragm and
the aperture iris diaphragm completely.
Caution in use of immersion oil:
If immersion oil enters your eyes or contacts your skin, immedi-
ately take the following treatment.
Eyes: Rinse with fresh water (for 15 minutes or more).
Skin : Rinse with water and soap.
If the appearance of the eyes or skin is altered or pain persists,
immediately see your doctor.
CAUTION
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CAUTION
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CAUTION
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Using Oil- or Water-Immersion Objective

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