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Olympus IX73 - Advanced Observation Methods; Phase Contrast Observation Procedures

Olympus IX73
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33
IX73
5-1 Phase Contrast Observation
} A phase contrast objective, phase contrast optical element, and the U-CT30-2 centering telescope are required for
phase contrast observation.
} If a DIC slider, analyzer or polarizer is engaged in the light path, disengage it.
With the IX3-LWUCDA or IX2-LWUCD
Optical Element Indication Applicable Objectives
IX-PHL (small) PhL UPLFLN4XPH
IX-PHC (small) PhC CPLN10XPH, LCACHN20XPH, CPLFLN10XPH
IX-PH1 (small) Ph1 UPLFLN10X2PH, UPLFLN20XPH, LUCPLFLN20XPH
IX-PH2 (small) Ph2 UCPLFLN20XPH, UPLFLN40XPH, LUCPLFLN40XPH, LUCPLFLN60XPH,
LCACHN40XPH
IX-PH3 (large) Ph3 PLAPON60XOPH, UPLFLN60XOIPH, UPLSAPO100XOPH, UPLFLN100XO2PH
Example of attaching optical elements (IX3-LWUCDA)
Small Diameter: PH1, PHL, free or PH2, C, free
Large Diameter: PH3, DIC40, DIC60, DIC100
With the IX-ULWCD
} The IX-PHCU or IX-PH1U can be attached only in the Ph1 and PhC. (Do not remove the built-in elements.)
Optical Element Indication Applicable Objectives
PHL (built-in) PhL UPLFLN4XPH
IX-PHCU PhC CPLN10XPH, LCACHN20XPH, CPLFLN10XPH
IX-PH1U Ph1 UPLFLN10XPH, UPLFLN20XPH, LUCPLFLN20XPH,
PH2 (built-in) Ph2 UCPLFLN20XPH, UPLFLN40XPH, LUCPLFLN40XPH, LUCPLFLN60XPH,
LCACHN40XPH
} When using the U-UCD8 or IX2-MLWCD, refer to the provided instructions.
} Insert the optical element (small) in the 30 mm position and the optical element (large) in the 38 mm position.
When observing the specimens in wells, it is recommended to use the IX-PHC to obtain the phase contrast effect
in a wide range of field of view.
5 OTHER OBSERVATION METHODS
1
Phase Contrast Optical Elements and Applicable Objectives

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