36
a
b
} Open the aperture iris diaphragm during phase contrast observa-
tion.
Engage the phase contrast objective in the light path and bring the
specimen into focus.
Remove an eyepiece and attach the U-CT30-2 centering telescope
in place.
Engage the ring slit of the condenser matching the phase contrast
objective in the light path.
Rotate the knurled section of the centering telescope to focus on
the ring slit
a
and the phase plate
b
of the objective.
Pushing the optical element centering knobs, turn the phase con-
trast ring slit centering screws (in positions marked) so that the ring
slit image overlaps with the phase plate of the objective.
} Do not release the hand suddenly while the optical element center-
ing knobs are being pushed in. The optical element centering
knobs may be popped out.
Remove the U-CT30-2 centering telescope and attach an eyepiece
in place.
} If the vessel is not completely flat, it may become necessary to
adjust the centering again to obtain the optimum contrast.
Adjust the centering in each objective power.
Adjust the field iris diaphragm so that its image circumscribes the
field of view and observe the phase contrast.
} Engaging the green filter in the light path will improve the contrast.
1
2
3
4
5
6
7
3
Centering the Phase Contrast Ring Slit
4 5
5
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