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In the formulae: PL indicates the number of cells falling in the lymphocyte region, PM the
number of cells falling in the mid-sized cell region, and PG the number of cells falling in the
granulocyte region. All three parameters are presented in 10
9
/L.
When the three percentages are obtained, the analyzer automatically proceeds to calculate the
lymphocyte number (Lym#), mid-sized cell number (Mid#) and granulocyte number (Gran#) by
below formulae, all parameters expressed in 10
9
/L.
Lym％, Mid％ and Gran％ are expressed in %, while WBC is in 10
9
/L.
White blood cell histogram
Besides the count results, the analyzer also provides a WBC histogram which shows the WBC
size distribution, with the x-axis representing the cell size (in fL) and the Y-axis representing
relative cell number (in 10
9
/L) (as shown below). The WBC histogram of a normal blood
sample (lysed and processed) should show display 3 clear parts: the small cell (about 20~70fl)
region represents the LYM group (lymphocytes); the mid-sized cell (about 70~150fl) region
represents the Mid group (including monocytes, eosinophils and basophiles); and the large
cell (over 150fl) region represents the Gran group (granulocytes).
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