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5.3
Introduction to Fluidic Channels
5.3.1 WBC/HGB channel
Reagent used: 1) cyanide-free lyse (break out RBCs and PLTs and 3-differentiate WBCs
based on cell size); 2) diluent (used for cleaning, and providing appropriate
environment for reaction and measurement)
Measurement principle: impedance method (for WBC count); colorimetric method
(for HGB measurement)
Measurement parameters: WBC and HGB
Output graph: WBC histogram
Dilution ratio: 1: 305.1 (whole blood mode); 1: 502 (pre-dilute mode)
Aperture diameter: 100um
Sample volume needed: 402.8μl; analysis time: 10s
Function description: blood sample and diluent are mixed in the WBC bath to get
diluted sample; then mixed 0.344ul of lyse. The sample solution after full reaction is
then sucked into the back bath by vacuum pressure in the vacuum chamber. The cells
are counted when they pass the aperture. Sample volume is calculated based on
analysis time.
5.3.2 RBC/PLT channel
Reagent used: diluent (dilution, cleaning, providing conductive environment and
processing cell sizes)
Measurement principle: impedance method
Measurement parameters: RBC and PLT
Graphics: RBC histogram; PLT histogram
Dilution ratio: 1: 16498.5 (whole blood mode); 1: 16449.4 (pre-dilute mode)
Aperture diameter: 70um
Sample volume needed: about 197.37μl; analysis time: 10s
Function description: sample probe aspirates diluted sample from the WBC bath, and
dispenses the sample as well as additional diluent to the RBC bath, where the sample
and the diluent are further mixed with existing initial volume of diluent. After full mixing
and reaction, the sample solution is then sucked into the back bath by vacuum pressure
in the vacuum chamber. The cells are counted when they pass the aperture. Sample
volume is calculated based on analysis time.
5.4
Sample Volume
Table 5-2 Sample Volume
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