18
Figure 3-1 Metering diagram
3.5.2 WBC-Related Parameters
White Blood Cell count
WBC (10
9
/L) is the number of leukocytes measured directly by counting the leukocytes
passing through the aperture.
Sometimes there are nucleated red blood cells (NRBC) presenting in the sample. While the
lyse will not be able to break their nuclear membrane, these NRBCs will also be counted as
WBCs. Therefore when NRBCs are found during microscopic exam, follow below formula to
modify the WBC count:
In the formula, WBC is corrected WBC count result; WBC is the WBC count provided by the
analyzer; and NRBC indicates the number of NRBCs found when every 100 WBCs are counted.
3-DIFF of WBC
Lyses and diluents change the sizes of each type of WBCs in various ways and at different
time. The WBCs are thus separated into 3 parts (from the largest size to the smallest):
lymphocytes, mid-sized cells (including monocytes, eosinophils, and basophils) and
granulocytes.
The analyzer then calculate the lymphocyte percentage (Lym%), mid-sized cell percentage
(Mid％) and granulocyte percentage (Gran%) (All presented in %) based on the WBC
histograms and in accordance with below formulae:
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