5 Maintenance
Nebulizer
Agilent 6475 Triple Quad LC/MS System User Guide 76
7 Loosen the fastener (locknut, 1.5-mm hex screw or T6 Torx screw) that
secures the needle holder in place.
8 Loosen the needle holder and pull it out of the nebulizer body. Dispose of the
used needle properly.
9 For more thorough cleaning, sonicate the nebulizer body. Pour isopropyl
alcohol or methanol through inside of the shaft.
10 Thread the new needle holder into the nebulizer body.
11 Carefully remove the new needle from the shipping tube and adhesive strip.
Identify the sharp end of the needle.
12 Carefully slide the sharp end of the needle through the ferrule until 1 cm of the
needle remains at the ferrule.
Do not bend the needle as you slide it into the ferrule. The needle is fragile. A
bent needle results in poor alignment with the nebulizer body.
13 Push the needle into the ferrule until the needle is slightly visible flush with the
ferrule.
14 Reinstall the locknut and the union. Hand-tighten the union.
15 Hold the needle holder steady with a 3-mm open-end wrench or 1/4-inch x
5/16-inch open-end wrench, depending on your nebulizer holder style.
16 Tighten the union one-half to three quarters of a turn, or until an audible
squeak is heard.
The ferrule is now compressed.
17 Pull carefully on the needle to ensure the needle is held firmly in place.
18 Insert the needle into the nebulizer shaft.
19 Adjust the electrospray needle position before reinstalling the nebulizer in the
spray chamber.
20 Tighten the fastener (T6 Torx screw) that secures the needle holder in place.
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