5 Maintenance
Nebulizer
Agilent 6475 Triple Quad LC/MS System User Guide 77
Installing the nebulizer
1 Insert the nebulizer part way into the spray chamber. 
Do not hit the tip of the needle as you insert the nebulizer. The tip of the needle 
is easily damaged. 
2 Connect the nebulizer sample line to the nebulizer. 
3 Turn the nebulizer clockwise and lock it in place.
4 Connect the sample nebulizer gas tubing to the nebulizer.
Do not over-tighten the LC fitting. An over-tightened fitting can crush the 
tubing, which creates a restriction.
Fixing Spray Stability EMF
If spray stability EMF is active, you should inspect, adjust or flush your nebulizer 
to ensure a stable spray from the CDS to the nebulizer. An unstable spray may 
lead to issues with tuning and sample analysis.
1 Inspect (page 73), adjust (page 75), or flush page 65) your nebulizer. If 
necessary, replace the nebulizer needle or nebulizer (page 75).
2 Follow the procedure to “Calibrating the LC/TQ (Checktune)” on page 30 or 
“Calibrating the LC/TQ (Autotune)” on page 31. The spray stability is 
reevaluated as part of a tune. If it within the acceptable range, the EMF 
warning should be removed following completion of the tune.