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Chapter 1: PlateLoc introduction

PlateLoc User Guide

Plate requirements

About this topic This topic explains the requirements for the plates you can use in the

PlateLoc.

Acceptable plates The PlateLoc accepts plates made from a variety of materials. For a

complete list of the acceptable materials, see the PlateLoc Thermal Plate

Sealer Seal Selection Guide. You can locate the guide on the Velocity11

website at www.velocity11.com.

Use only plates that meet the standards established by the Society of

Biomolecular Sciences (SBS), including deep-well, PCR, and standard

plates in the 96-well, 384-well, and 1536-well formats. For the latest plate

standards, go to www.sbsonline.org. You can also contact the labware

manufacturer to inquire about SBS-standard plates.

Ideal seal conditions can depend on the plate design, such as thickness

and geometry. For example, plates with raised chimneys that have flat

surfaces at the top can minimize cross-contamination, reduce

evaporation, and minimize condensation.

Note: Plates made from the same material but have different designs can

require different sealing parameters.

Velocity11 recommends that you run optimization tests to determine the

best sealing parameters to use for each plate type. See “Optimizing seal

quality” on page 77 for some optimization test guidelines.

Challenging plates Because of the material or design, the following plates might cause poor

seal results when used in the PlateLoc:

❑ Plates that have non-binding coatings

❑ Flexible plates that tend to bend during sealing

❑ Breiner Model 651182, 96-well V-bottom plates (when used with the

Velocity11 Peelable Aluminum Seal, 06643.001)

You should run some sealing tests to determine whether optimal sealing

is possible with these plates or if you should use different plate types.

See “Optimizing seal quality” on page 77 for some optimization test

guidelines.

Agilent Technologies PlateLoc User Manual

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