Applied Biosystems 7500 - Page 149

Index
125
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
omit wells 90
online Help. See Help system
order materials 37
other fluorescent-based reagents 10
OUTLIERRG flag 89
outliers. See omit wells.
overvoltage category (rating) xx
P
physical hazard safety xx
prepare experiment
for more information 46
, 50, 54
guidelines 46
, 48, 50, 53
reaction mix
prepare 49
reaction plate 51
sample dilutions 45
standard dilution series 47
workflow 44
prepare for run 57
print reaction setup instructions 35
publish data 83
Q
QC Summary screen 88
QuickStart 11
, 100
R
radioactive waste, handling xix
ramp speed 22
, 23
Raw Data Plot screen 94
reaction mix
calculated volumes 33
volumes 49
reaction plate
layout 13
, 40
load 57
prepare 51
unload from the instrument 64
reaction plates 6
Reaction Setup screen 32
reagents
other fluorescent-based 10
SYBR Green 9
TaqMan 9
repetitive motion, safety xxi
replicate, component of experiment 7
results, interpreting 67
run experiment
alerts 63
enable notification settings 59
for more information 63
guidelines 60
monitor 61
prepare for 57
start 61
workflow 56
Run Method library 31
Run Method screen 30
monitor during a run 63
S
safety
before operating the instrument xvi
biological hazards xxi
chemical xvii
chemical waste xix
conventions xii
electrical xx
ergonomic xxi
guidelines xviii
, xix
instrument operation xvi
moving and lifting instrument xvi
moving parts xx
moving/lifting xvi
physical hazard xx
repetitive motion xxi
standards xxii
workstation xxi
safety labels, on instruments xiv
safety standards xxii
safety symbols, on instruments xiii
sample dilutions
calculated volumes 34
, 45
prepare 45
Sample screen 28
samples
design guidelines 29
dilutions 45
sample reactions (unknowns) 53
set up 28
select wells 69
singleplex PCR 8
, 24
SPIKE flag 89
standard curve experiments
about 7
standard deviation, effect of threshold on 79
standard dilution series
calculated volumes 34
component of experiment 7
prepare 47
standard vs. fast plates 6
standards
component of experiment 7
design guidelines 27
diluting 47
EMC xxii
safety xxii
set up 26