Index
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
126
Set Up Standards check box 24
, 25
standard reactions 52
Standards screen 26
SYBR Green reagents 3
, 9, 23, 24, 25, 36, 95
symbols, safety xiii
T
TaqMan reagents 3, 9, 23, 24, 25, 36, 95
targets
design guidelines 25
set up 24
Targets screen 24
Technical Support, contacting xi
Template 11
, 102
template. See samples.
text conventions viii
THOLDFAIL flag 89
threshold
correct values 78
examples 79
manually adjust 87
training, information on xi
troubleshooting
adjust baseline 87
adjust threshold 87
flags 89
omit wells 90
view analysis settings 86
view Multicomponent Plot screen 92
view QC Summary screen 88
view Raw Data Plot screen 94
U
unload instrument 64
user attention words, described viii
using this guide
as a tutorial 11
with your own experiments 11
W
WARNING, description xii
waste disposal, guidelines xix
Well Table 81
wells
negative control 28
, 41, 51
omit 90
selecting 69
standard 28
, 41, 51
unknown 28, 41, 51
workflows
Advanced Setup 98
example experiment 14
Export/Import 11
, 104
QuickStart 11, 100
Template 11
, 102
workstation safety xxi