Overview 3
STERRAD VELOCITY Reader User’s Guide 13
Figure 4. STERRAD VELOCITY Biological Indicator / Process Challenge Device
Biological Indicator Wells
There are eight individual biological indicator incubation wells at the top along the
back of the device. Positioned in the bottom of the wells is a heater block which maintains
a constant temperature range to promote the growth of the indicator organism. If any of
the indicator organisms survive the sterilization cycle they will produce an enzyme which
reacts with the growth medium causing it to uoresce under ultraviolet light. Each well
contains an ultraviolet light source to excite uorescence in the growth medium, and a
photodetector to detect that uorescence.
Directly under each well is a well number illuminated by a well status indicator
light. The light can shift between three colors (white, green, and red). The colors and
states are explained in the table below.
Well Status Indicator Light
Indicator State Meaning
Off Well Open
Solid White Adding BI to Well
Solid Green Passed Result
Solid Red Failed Result
Table 2. Well Status Indicator
Growth
Reservoir
Spore Disc
Insert
Medium Ampoule
Vial
Sterilant
Ingress
Window
BI Cap
with Label
Chemical Indicator
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