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Operation of the Liberty Blue
Creating a New Liberty Method
1. Open the appropriate folder or sub folder in the Methods box for the new method.
2. Click the New Method button.
3. The method will appear in the appropriate folder. Enter a name for the method and press Enter. Click into the
Sequence box. A list of the amino acids in the current bottle setup will drop down. To enter the sequence, either
click the appropriate amino acid positions in the drop-down, or type the one-letter abbreviation for the desired
amino acids.
NOTE
To delete an amino acid from the sequence, press the backspace key (to delete the residue before the
cursor) or click the delete button on the drop-down (to delete the highlighted residue).
To insert an amino acid into the sequence, click an amino aid in the sequence to highlight it, then either
click the desired amino acid in the drop-down, or type the one-letter abbreviation for the desired amino
acid. The new residue will be inserted before the highlighted amino acid.
4. Select the scale (0.005 mmol to 5.0 mmol) from the Synthesis Scale box, then select the resin swelling type
(Standard or High Swelling) from the Resin Swelling box.
NOTE
The scale and resin type must be selected before changing any other parameters, as each scale and resin
type has specic default options that will be loaded upon selection.
5. The remaining options can now be selected.
5.1. C-Terminus: The C-terminus (acid or amide) is only used to calculate the molecular weight, and does
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