cytiva BPG Columns - Page 117

Media Contaminants Suggested procedure Follow-up
Lipids Wash with 2 to 4 column volumes of
0
.5 to 2 M NaOH for a contact time of
1 to 2 hours. Use reversed flow, or
wash with 2 to 4 column volumes of
0.1% to 0.5% non-ionic detergent for a
contact time of 1 to 2 hours. Use
reversed flow, or
wash with 2 to 4 column volumes of
30% isopropanol or 70% ethanol for a
contact time of 1 to 2 hours. Use
reversed flow.
Regenerate to start
c
onditions. See
instructions for
respective media.
Sephacryl High
R
esolution,
Sephadex
G-25
Precipitated
proteins/lipids.
Wash with 0.5 to 1 column volumes of
0
.5 M NaOH for a contact time of
0.5 to 1 h. Use reversed flow, or
wash with 2 to 4 column volumes of
0.5% non-ionic detergent for a contact
time of 1 to 2 hours. Use reversed flow.
Wash immediately
w
ith at least 2
column volumes of
working buffer.
Protein A
S
epharose 4
Fast Flow,
Protein G
Sepharose 4
Fast Flow
Precipitated or
hydrophobically
bound
substances
Wash with 1 to 2 column volumes of 6
M Guanidine-HCl for a contact time of
1 to 2 hours. Use reversed flow, or
wash with 2 to 4 column volumes of
0.1% to 0.5% non-ionic detergent for a
contact time of 1 to 2 hours. Use
reversed flow.
Wash immediately
with at least 5
column volumes of
working buffer.
Superdex 75
a
nd 200 prep
grade
Precipitated
proteins/lipids.
Wash with 0.5 to 1 column volume of
0.5 M NaOH for a contact time of
0.5 to 1 hour. Use reversed flow, or
wash with 2 to 4 column volumes of
0.1% to 0.5%non-ionic detergent for a
contact time of 1 to 2 hours. Use
reversed flow.
Wash immediately
with at least 2
column volumes of
working buffer.
After cleaning
A
fter cleaning, restore the column to working conditions using the follow-up proce-
dure. All conditions refer to room temperature unless otherwise stated. If special main-
tenance routines are required, contact your local Cytiva representative for expert
advice. For affinity media, please contact your local Cytiva representative.
6 Maintenance
6
.7 Cleaning
BPG Columns Operating Instructions 29193223 AB 117