5.1.2 Slurry preparation
S
lurry concentration
Mix the packing buffer with the media to form a 50% slurry, i.e., sediment volume/total
slurry volume = 0.50. This slurry volume is appropriate for BioProcess media.
Make sure that the correct amount of slurry is used to end up with the target bed
height and optimal bed compression.
More information regarding how to determine slurry concentration using the Slurry
Concentration Kit can be found in Instruction 29112530.
Compression factor
The media needs to be compressed slightly to maintain a stable packed bed. The multi-
plication factor to convert packed bed volume to settled media volume is the compres-
sion factor.
Compression Factor CF = (gravity settled bed height)/(packed bed height)
Compression factor should read for most Cytiva media, between 1.10 and 1.15.
Amount of media and slurry volume
The amount of media required for packing the column can be calculated using the
following formula:
A = (π × r2 × H × Cf/1000)
Where:
A = volume of media needed, liter
π = 3.14
r = radius of column (half the diameter), cm
H = bed height in column, cm
Cf = Compression factor of media
The 50% slurry volume is calculated as: A/0.5
Media quantities required per cm bed
height
The table shows the amount of media per centimeter of packed bed. The compression
factor is 1.15.
BPG column Column diameter
(
mm)
(π × r2 × H × Cf/1000) Amount of media
1
per cm of
packed bed
BPG 100 100 78.5 × 1.15 90.3 ml
BPG 140 140 154 × 1.15 177 ml
5 Operation
5
.1 Preparation
5.1.2 Slurry preparation
50
BPG Columns Operating Instructions 29193223 AB
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