5.1 Preparation
A
bout this section
This section contains information how to prepare the BPG columns before column
packing. Information and support related to optimal column packing can be obtained
from your Cytiva representative.
In this section
Section See page
5.1.1 Column flow packing method 49
5.1.2 Slurry preparation 50
Location
T
ake care in selecting the position of the column before proceeding. System, tanks,
drains and handling devices for the larger columns all require space and easy, safe
access during all operations of the column. Take care to route the hoses carefully to
avoid tripping risks and always try to keep hose lengths to a minimum.
Arrange connections
NOTICE
O
nly use tubing and clamps approved for the maximum pressure of
the column and resistant to the chemicals intended to be used.
Make sure the tubing inner diameter is constant throughout your system setup to
m
inimize unwanted volume and keep a consistent flow and pressure in the process
stream. The valves, tubing and gaskets used should have the same inner diameter as
the column outlets or slightly larger
Materials
The following materials are suitable for the column packing:
• BPG column with suitable net for the media
• Media about 50% slurry in water
• WFI for packing
5 Operation
5
.1 Preparation
BPG Columns Operating Instructions 29193223 AB 47
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