5.2.5 Storage and unpacking of packed columns
S
torage
Both outlets of the column should be sealed after equilibrating the column in storage
buffer. The column must be stored in a temperature-controlled environment with
water or 20% ethanol above the adapter O-ring to prevent it from drying out and
cracking. Covering the column with a clean plastic bag helps prevent dust and debris
from accumulating on exterior surfaces.
Unpacking
Place several centimeters of buffer or water above the adapter O-ring of the BPG
column and fully loosen the wing nut which seals the adapter O-ring. Make sure the
adapter outlet is open to waste. If the adapter is not easily raised, try slightly lowering it
to break the O-ring seal on the glass tube. If the O-ring rolls, tighten the wing nut a bit
to prevent this during adapter ascent. A firm twist on the threaded adapter shaft is also
useful when attempting to unseal the O-ring.
Raise and remove the adapter. If the packed bed is difficult to reslurry, pumping buffer
up through the bottom valve will help to lift and disperse the media more rapidly and
clear the bottom net.
5 Operation
5
.2 Column packing
5.2.5 Storage and unpacking of packed columns
70
BPG Columns Operating Instructions 29193223 AB
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