6.2 Replacements of nets and O-rings
I
n this section
Section See page
6.2.1 Replacements in the adapter 81
6.2.2 Replacements in the column 87
Nets
B
lockage of the nets causes malfunction of the columns and an over-used net can
affect distribution. To prevent blockage follow the instructions listed below.
• When columns are to be re-packed, always check the nets and wash them in a
detergent solution or replace them.
• It is important to select the appropriate adapter and end-piece nets, see Recom-
mended net sizes, on page 48.
• Check the HETP and A
s
regularly to prevent poor performance due to old nets.
O-rings and seals
The sealing O-rings may lose their flexibility with time and need to be replaced regu-
larly. Worn O-rings may not seal properly.
Tip:
Cytiva recommends that column O-rings and seals are changed at least
o
nce each year. It may be necessary to change O-rings and seals exposed to
some form of movement in the column more frequently, for example the O-
rings on the adapter.
Liquids required for changing nets
a
nd O-rings
• 100% ethanol for cleaning
• 20% ethanol for lubricating seals
• Warm water (approximately 50°C)
6 Maintenance
6
.2 Replacements of nets and O-rings
BPG Columns Operating Instructions 29193223 AB 79
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