5 Operation
A
bout this chapter
BPG columns can be used to perform a number of different chromatographic purifica-
tions, depending on the chromatography media and specific methods selected for use.
This chapter provides general instructions on how to prepare and operate BPG
columns.
In this chapter
Section See page
5.1 Preparation 47
5.2 Column packing 53
5.3 Performance evaluation of the column 72
Precautions
WARNING
O
nly properly trained personnel may operate and maintain the
product.
WARNING
T
horoughly check the glass column tube to make sure that it is not
cracked or has been severely scratched. Pay extra attention to the
end parts of the column tube. The pressure specifications are only
valid if the glass tube is undamaged.
WARNING
T
here should NEVER be air or gas under pressure in the column.
5 Operation
BPG Columns Operating Instructions 29193223 AB 45
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