5.2.4 Packing the column - second step
D
escription of the second step
The second step of flow packing is to compress the bed using a flow rate predeter-
mined from the pressure flow curve data, and rapidly set the adapter position to main-
tain the packed bed density. The pump is adjusted quickly to the desired flow rate to
obtain the final bed height, and the integrity of the adapter O-ring seal checked and
further tightened if necessary. When the bed has stabilized in about 1 CV, the bed
height is marked on the column tube. The top valve is turned to place the pump out of
line with the column at the same time as the bottom column valve is closed.
The adapter must be rapidly lowered into place with minimal disturbance of the packed
bed. This should be done with the adapter O-ring just loose enough that buffer exits out
of the top valve and not around the O-ring. Once the adapter is in place, the O-ring
should be retightened immediately. If the packed bed decompresses significantly
above the marked bed height before the adapter can be lowered in place, this second
packing step can be repeated once. Wait at least 5 minutes for the bed to cure before
moving the column.
Second step
Step Action
1 Open the bottom valve and put the top valve from pump to column (A to B)
a
nd start the pump. This will move the bed down and once stabilized the
level should be marked on the tube.
2 Stop the pump.
5 Operation
5
.2 Column packing
5.2.4 Packing the column - second step
BPG Columns Operating Instructions 29193223 AB 67
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