cytiva BPG Columns - Page 68

Step Action
3 When the pressure reaches zero, position the top valve to waste (B to C), and
move the adapter down, pushing into the media until the adapter reaches
the mark on the tube.
A
B
C
4 Close the top valve and the bottom valve.
5
Result:
T
he column is now packed.
6 Test the packed column, see S
ection 5.3 Performance evaluation of the
column, on page 72.
5 Operation
5
.2 Column packing
5.2.4 Packing the column - second step
68
BPG Columns Operating Instructions 29193223 AB