5.2.1 Preparing the column
C
olumn preparation
Operation of the adapter, integrity of the adapter O-ring and porosity of the support
nets should be checked before starting. The column tube and all internal surfaces must
be clean.
Remove air from bottom net
Every effort must be made to remove air from bottom net and tubing. Air is removed
from under the bottom net using suction and about 1 cm buffer left in the column.
Step Action
1 Make sure that the column is level, see S
ection 4.4 How to level the column,
on page 38.
2 Make sure that the column is grounded, see Section 4.5 Grounding kit, on
page 40.
3 Wet the column net with 20% ethanol and pour 2 to 3 cm of packing buffer
into the column with the bottom valve closed and the outlet to drain.
5 Operation
5
.2 Column packing
5.2.1 Preparing the column
54
BPG Columns Operating Instructions 29193223 AB
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