BPG column Column diameter
(
mm)
(π × r2 × H × Cf/1000) Amount of media
1
per cm of
packed bed
BPG 200 200 314 × 1.15 361 ml
BPG 300 296 0.707 x 1.15 812 ml
BPG 450 446 1.59 x 1.15 1.83 l
1
DEAE Sepharose™ FF, Q Sepharose FF, CM Sepharose FF, S Sepharose FF, Phenyl Sepharose FF, Sepharose 4 FF and
6 FF, Sephacryl™ High Resolution, Superdex™ 75 and 200 prep grade, Q and SP Sepharose HP, Phenyl Sepharose HP
Sephadex G-25
The Sephadex™ G25 media swell 4 to 5 times (1 g = 4-5 ml of media). The table shows
the amount of media per centimeter of packed bed.
Note:
The figures in the table below are based on four-fold swelling and that there
c
an be batch-to-batch variation.
Media Amount of
m
edia per
liter of
packed
column
(g)
Pack
f
actor
(PF)
(l)
Amount of media per cm of packed bed (g)
BPG
1
00
BPG
140
BPG
200
BPG
300
BPG
450
Sephadex G-25
C
and M grades
250 1.15 l 22.6 43.9 90.3 202.4 453
Sephadex G-25
g
rade SF
280 1.15 l 22.6 43.9 90.3 202.4 453
5 Operation
5
.1 Preparation
5.1.2 Slurry preparation
BPG Columns Operating Instructions 29193223 AB 51
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