Problem Cause Action
There have been any changes to the
f
ollowing:
• Preparation of buffers/solutions
• Preparation of system and tubing
• C
heck the dilution, filtration,
temperature and prepara-
tion of the sample and
buffers are considered.
• Check all dead volumes were
correctly filled with appro-
priate buffers and sample
before running.
Infected and/or
p
yrogen contami-
nated column
Column bed shows signs of bacterial
growth.
• C
IP.
• Prepare new buffers/sample.
The starting material is contaminated. Use clean material.
The in going components (such as water,
s
erum components in growth media, etc.)
are not ok.
Check material.
The column is not cleaned. Clean the column, refer to
R
ecommended CIP procedure,
on page 116.
The NaOH has been removed, in case that
p
ositive LAL tests were seen after a CIP
cycle with NaOH.
Repeat cleaning.
Air trapped in the
c
olumn
The buffers have not the same tempera-
ture as the column.
Check the buffers are at the
same temperature as the
column.
The connections are not correctly fitted
t
ogether.
Check the connections.
The valves are not shut tight enough. Check the valves and close
t
hem.
The volume of the trap is too small or large.
• F
ill air trap with buffer.
• Change air trap.
7 Troubleshooting
BPG Columns Operating Instructions 29193223 AB 123
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