Column material
T
he materials used in the BPG columns are compatible with the solvents most
commonly used in chromatography separations of biomolecules, column mainte-
nance and cleaning processes. All polymeric materials in contact with the process
stream meet the requirements for USP class VI, described in USP <88> Biological Reac-
tivity Tests, In Vivo.
Adapter and rods
The adapter top plate and the adjuster nut are bolted to the column flange. The
adjuster nut is allowed to rotate on a bushing ring in the adapter top plate, altering the
height of the adapter plate in the column tube.
The adapter O-ring forms a seal between the adapter and the glass column when it is
compressed by the sealing unit. This is controlled by the seal adjuster knob.
The rods hold the flange and end-piece to the glass tube which is sealed by O-rings. The
O-rings effectively isolate the glass tube from direct contact with the stainless steel
components.
Nets
The column is delivered with 23 μm (PolyPropylene) nets. For media with an average
particle diameter <70 μm, change to 10 μm (Polyamide), or 12 μm (PEEK) in both
adapters and end-pieces. For big beads use 54 μm nets, see Recommended net sizes,
on page 48.
Connections
BPG columns can be connected to different types of tubing and systems. To protect
the packed bed it is advisable to have isolation valves on both the inlets and outlets of
the column.
3 Column descriptions
3
.1 Overview
20
BPG Columns Operating Instructions 29193223 AB
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