cytiva BPG Columns - Page 65

Step Action
7 Lower the adapter quickly by turning the height adjuster handle clockwise.
The buffer between the sedimented bed and the adapter passes between
the adapter O-ring and the column wall. During this operation the bed will
start to rise. Stop lowering the adapter when it is 0.5 to 1.0 cm above the bed.
0.5 - 1.0 cm
A
B
C
8 Seal the adapter O-ring by turning the knob, or seal adjuster handle, clock-
wise and lower the adapter to 0.1 or 0.2 cm above the bed.
A
B
C
0.1 - 0.2 cm
5 Operation
5
.2 Column packing
5.2.3 Packing the column - first step
BPG Columns Operating Instructions 29193223 AB 65