TABLE OF CONTENTS
1. INTRODUCTION ........................................................................................................................................ 7
1.1. NAME AND SERIAL NUMBER .................................................................................................................... 7
1.2. INTENDED USE ........................................................................................................................................ 7
1.3. INTEGRATED SOFTWARE ......................................................................................................................... 8
2. FUNCTIONAL DESCRIPTION ................................................................................................................. 9
2.1. MAIN ELECTRONIC PARTS OF THE ANALYZER .......................................................................................... 9
2.1.1. Counting chambers with electrodes and measuring apertures ................................................... 10
2.1.2. HGB Head ................................................................................................................................... 10
2.1.3. Cell counter Amplifier Board ...................................................................................................... 11
2.1.4. Control and Measurement Board (COMB) with DIMM-PC core ............................................... 12
2.1.5. DIMM-PC* Module .................................................................................................................... 13
2.1.6. Configuration and ID E
2
PROM board (IDEPROM) .................................................................. 13
2.1.7. Pneumatic and Power Board (PPB) ........................................................................................... 14
2.1.8. Opto-boards for stepper motors .................................................................................................. 15
2.1.9. Valve boards ............................................................................................................................... 15
2.1.10. Pressure Sensor ........................................................................................................................... 16
2.1.11. Digital Reagent Sensor Board..................................................................................................... 16
2.1.12. LCD Display Module with High Voltage Board ......................................................................... 17
2.1.13. Keypad ........................................................................................................................................ 18
2.1.14. External Power Supply ................................................................................................................ 18
2.2. MAIN MECHANIC AND FLUIDIC PARTS OF THE ANALYZER ..................................................................... 19
2.2.1. Sampling needle .......................................................................................................................... 20
2.2.2. Washing head .............................................................................................................................. 20
2.2.3. H&V moving unit ........................................................................................................................ 21
2.2.4. Main Dilutor................................................................................................................................ 22
2.2.5. Micro Dilutor .............................................................................................................................. 23
2.2.6. Puffer reservoir ........................................................................................................................... 23
2.2.7. Pump ........................................................................................................................................... 23
2.3. ASSEMBLED ANALYZER ........................................................................................................................ 24
2.3.1. Abacus+ ...................................................................................................................................... 24
3. ADJUSTMENT ........................................................................................................................................... 27
3.1. MECHANICAL SETTINGS ........................................................................................................................ 27
3.1.1. Opto wheel setting ....................................................................................................................... 27
3.1.2. Sampling needle setting ............................................................................................................... 28
3.2. HARDWARE SETTINGS ........................................................................................................................... 28
3.2.1. Amplifier offset setting ................................................................................................................ 28
4. OPERATION OF THE FLUIDIC SYSTEM ........................................................................................... 29
4.1. BASIC PROCESSES ................................................................................................................................. 30
4.1.1. Sampling process ........................................................................................................................ 30
4.1.2. Needle washing process .............................................................................................................. 31
4.1.3. Diluting processes ....................................................................................................................... 32
4.1.4. Fluid transfer from MIX chamber to WBC chamber ................................................................... 33
4.1.5. Lysing process ............................................................................................................................. 34
4.1.6. Counting process ......................................................................................................................... 35
4.1.7. Aperture priming process ............................................................................................................ 36
4.1.8. WBC chamber draining process .................................................................................................. 37
4.1.9. RBC chamber draining process .................................................................................................. 38
4.1.10. Cleaner priming process ............................................................................................................. 39
4.2. MAIN FUNCTIONS OF FLUIDIC SYSTEM ................................................................................................... 40
4.2.1. Initialization ................................................................................................................................ 40
4.2.2. Wake up ....................................................................................................................................... 40
4.2.3. Measurement cycle ...................................................................................................................... 41
4.2.4. Standby ........................................................................................................................................ 47
4.2.5. Cleaning ...................................................................................................................................... 48
4.2.6. Hard cleaning ............................................................................................................................. 48
4.2.7. Shutdown ..................................................................................................................................... 48
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