4.2.5. Cleaning
Cleaning function drains all of the chambers and fills the RBC chamber and the MIX
chamber with 2.5 ml of diluent. After moving content of the MIX chamber into the WBC
chamber the aperture branch is primed with cleaner (see 1.x). The remaining cleaner
between the cleaner detector and the V6 valve is pushed into the MIX chamber with
diluent. After this the WBC chamber is drained and the cleaner with diluent transferred
here from the MIX chamber. Then the apertures are cleaned with high voltage burn and
with backflush. The RBC chamber rinsed with 5.0 ml of diluent and the remaining cleaner
is pushed out with diluent from the aperture branch (and back side off the apertures). At
least the volumes of the standby state are dosed into the chambers.
4.2.6. Hard cleaning
Before the hard cleaning a wakeup procedure is executed. After pressing the start button
the M3 dilutor aspirates 0.2 ml from the hard cleaning solution. Half of this amount is
dosed into the MIX chamber (diluted with 5.0ml diluent coming from the other dilutor unit
M4 through V10, V8 , V6) and the remaining cleaning solution is pushed into the RBC
chamber with 5.0 ml diluent (see diluting process). Then the M4 dilutor unit pulls and
pushes the diluted cleaner solution via the aperture combined with high voltage burns.
After this the chambers are drained and rinsed with diluent.
4.2.7. Shutdown
The fluidic shutdown performs the following steps:
Drains chambers
Perform a cleaning cycle
Priming chamber with diluent to avoid drying out of aperture (MIX 2.0 ml, RBC 5.0 ml,
WBC 5.0 ml)
Sampling needle is positioned above MIX chamber, needle up
All of the syringes are positioned down
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