Dionex UltiMate 3000 - Fluidic Setup; Installation Tips

UltiMate 3000 RSLCnano – Standard Applications
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2.4.2 Fluidic Setup
Figure 10 presents the setup using the parts of the Direct Injection application kit. Columns
are marked with letters, tubing with digits, and the sample loop is installed in the WPS-
3000PL Autosampler
Figure 10: Fluidic connections for a Direct Injection experiment onto a nano column
Table 2: UltiMate 3000 RSLCnano Direct Injection nano LC kit (P/N 6720.0300) contents
# Item Replacement P/N
a
75 µm I.D. x 15 cm, packed with Acclaim PepMap RSLC C18, 2 µm,
100Å, nanoViper
164534
1
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 20 µm x 750 mm 6041.5280
2
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 20 µm x 550 mm 6041.5260
nanoViper sample loop 1 µL, FS/PEEK sheathed I.D. x L 100 µm x 127 mm 6826.2401
Polypropylene vials for WPS with glass insert, 250 µL, 25 pcs. 6820.0027
Polypropylene caps for WPS vials, 25 pcs. 6820.0028
Cytochrome C digest, 1.6 nmol, Lyophilized 161089
2.4.3 Installation Tips
• Follow the General Recommendations for Applications (→ page 6).
• The impact of dwell (dead) volumes on reproducibility is very important. Improper
connections of the different elements are the most likely cause of failure for this
application.
Tip: When no valve is
available, a union can be used to
connect the capillary column
(P/N 6040.2304)