UltiMate 3000 RSLCnano – Standard Applications
54
4 Appendix – Traditional Capillary Connections
• When installing fused silica tubing (for example, the capillary column):
o Insert the PEEK sleeve with the stainless steel nut (standard) or PEEK nut
(biocompatible system) and the appropriate ferrule in a port (or the preassembly
tool, when working on a biocompatible system!).
o Push the sleeve all the way into the port. It is essential for a zero dead volume
connection that the assembly seats firmly.
o Tighten the nut by two or three turns to make certain that the ferrule grabs the
sleeve.
o Remove the pre-assembled fitting, and then slide the capillary (column) into the
sleeve.
o When re-installing the fitting together with the capillary (column), make certain
that the tip of the tubing does not extend the tip of the sleeve. Tighten the nut
finger-tight.
o Push the capillary (column) all the way into the sleeve (port) to minimize dead
volume. It is essential for a zero dead volume connection that the assembly seats
firmly.
• To prepare connections with PTFE sleeves:
o Start flow delivery.
o Slide the fused silica capillary (for example, the column) approximately 1- 2 mm
into the sleeve.
o Wait until a droplet is formed at the outlet. Remove the droplet (together with
potential dirt in the tubing).
o Now push the capillary (column) half-way into the sleeve. Wait again until a
droplet is formed and remove it.
o Connect the second capillary (for example, the inlet capillary of the flow cell).
To Next Page
Loading...
