UltiMate 3000 RSLCnano – Standard Applications
8
2.1.3 Mobile Phases
• Always use fresh solvents.
• When running online multidimensional approaches, make sure that the mobile phase of
the first dimension will allow for trapping the sample on the (trap) column used on the
second dimension.
• For example, do not use phosphate buffers from SCX separations as loading solvent in
RP separations.
• Thermo Fischer Scientific recommends replacing (aqueous) solvents at least once every
two weeks.
Tip: Replace solvents completely; do not ’top up’ to avoid unwanted components
building up in the mobile phases.
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