Dionex UltiMate 3000 - Manual Injection (Basic Configuration)

UltiMate 3000 RSLCnano – Standard Applications
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2.5.2 Fluidic Setup
Figure 13 presents the setup using the parts of the Direct Injection application kit. Columns
are marked with letters, tubing with digits, and the sample loop is installed in the
WPS-3000PL Autosampler.
Figure 13: Fluidic connections for a Direct Injection experiment onto a capillary column
Table 3: UltiMate 3000 RSLCnano Direct Injection capillary LC Kit (P/N 6720.0305) contents
# Item Replacement P/N
a
300 µm I.D. x 15 cm, packed with Acclaim PepMap RSLC C18, 2 µm,
100Å, nanoViper
164537
1
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 50 µm x 750 mm 6041.5580
2
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 50 µm x 550 mm 6041.5560
nanoViper sample loop 5 µL, FS/PEEK sheathed I.D. x L 200 µm x 159 mm 6826.2405
Polypropylene vials for WPS with glass insert, 250 µL, 25 pcs. 6820.0027
Polypropylene caps for WPS vials, 25 pcs. 6820.0028
Cytochrome C digest, 1.6 nmol, Lyophilized 161089
Tip: When no valve is
available, a union can be used to
connect the capillary column
(P/N 6040.2304)