Dionex UltiMate 3000 - Default Chapter; Table of Contents

UltiMate 3000 RSLCnano – Standard Applications
2
Table of Contents
1 Introduction ................................................................................................................ 4
1.1 Additional Documents ............................................................................................... 5
2 Application Setup ....................................................................................................... 6
2.1 General Recommendations for Applications............................................................. 6
2.1.1 Connections ........................................................................................................ 6
2.1.2 Sample Preparation ............................................................................................. 7
2.1.3 Mobile Phases ..................................................................................................... 8
2.2 Installing the UltiMate 3000 RSLCnano system ....................................................... 9
2.3 Application Overview ............................................................................................. 12
2.4 Direct Injection onto a Nano Column ..................................................................... 13
2.4.1 Hardware Layout .............................................................................................. 13
2.4.2 Fluidic Setup ..................................................................................................... 15
2.4.3 Installation Tips ................................................................................................ 15
2.4.4 Testing the Application .................................................................................... 16
2.4.5 Large Volume Injections .................................................................................. 16
2.5 Direct Injection onto a Capillary Column ............................................................... 18
2.5.1 Hardware Layout .............................................................................................. 18
2.5.2 Fluidic Setup ..................................................................................................... 19
2.5.3 Installation Tips ................................................................................................ 20
2.5.4 Testing the Application .................................................................................... 20
2.6 Pre-concentration onto a Nano Column .................................................................. 21
2.6.1 Hardware Layout .............................................................................................. 21
2.6.2 Fluidic Setup ..................................................................................................... 22
2.6.3 Installation Tips ................................................................................................ 23
2.6.4 Testing the Application .................................................................................... 23
2.7 Pre-concentration onto a 200 µm Monolithic Column ............................................ 25
2.7.1 Hardware Layout .............................................................................................. 25
2.7.2 Fluidic Setup ..................................................................................................... 26
2.7.3 Installation Tips ................................................................................................ 27
2.7.4 Testing the Application .................................................................................... 27
2.8 Pre-concentration on a Capillary Column ............................................................... 29
2.8.1 Hardware Layout .............................................................................................. 29
2.8.2 Fluidic Setup ..................................................................................................... 30
2.8.3 Installation Tips ................................................................................................ 31
2.8.4 Testing the Application .................................................................................... 31
2.9 2D Salt Steps with Nano Column ............................................................................ 32
2.9.1 Hardware Layout .............................................................................................. 32
2.9.2 Fluidic Setup ..................................................................................................... 33
2.9.3 Installation Tips ................................................................................................ 34
2.9.4 Testing the Application .................................................................................... 35
2.9.5 Salt Solutions Preparation ................................................................................ 36
2.10 Automated off-line 2D LC of Peptides, micro SCX x nano RP .............................. 37
2.10.1 Hardware Layout .............................................................................................. 37
2.10.2 Fluidic Setup ..................................................................................................... 39
2.10.3 Installation Tips ................................................................................................ 40
2.10.4 Testing the Application .................................................................................... 41
2.11 Automated off-line 2D LC, Cap RP (basic) x nan RP (acidic) ............................... 42
2.11.1 Hardware Layout .............................................................................................. 42
2.11.2 Fluidic Setup ..................................................................................................... 43