UltiMate 3000 RSLCnano – Standard Applications
40
Table 8: UltiMate 3000 RSLCnano Automated Off-line SCX-RP peptides kit (P/N 6720.0330) contents -
Continued
# Item Replacement P/N
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 20 µm x 750 mm 6041.5280
6
nanoViper sample loop 50 µL, FS/PEEK sheathed I.D. x L 250 µm x 408 mm 6826.2450
7
PTFE tubing, 500 µm I.D. 100 cm, used as waste tubing 6720.0077
Protein mixture digest, 100 pmol, Lyophilized 161088
Cytochrome C digest, 1.6 nmol, Lyophilized 161089
Polypropylene vials for WPS with glass insert, 250 µL, 25 pcs. 6820.0027
Polypropylene caps for WPS vials, 25 pcs. 6820.0028
96 Well Microplate, PP, V-Bottom 6820.4113
1/16" Universal Fingertight Fitting, one-piece design, extra long thread, 4 pcs. 6720.0015
Buffer tubing 500 µL, WPS-3000PL 6820.0020
LCi Solutions Library CD 6830.0400
Operating Instructions for automated off-line 2D-LC of peptides and proteins 164208
2.10.3 Installation Tips
• Follow the General Recommendations for Applications (→ page 6).
• The design of the nano trap column provides the easiest connections, but must only be
used in forward flush operation. Please check the indicated flow direction when
installing a nano trap column.
• If the loss of hydrophilic peptides is observed, the concentration of acetonitrile in the
loading solvent can be decreased down to 99/1 water/ACN + 0.05% TFA
• If too much hydrophobic secondary interaction is observed on the IEX column, the
amount of ACN can be increased up to 5% or 10%.
• The loading time and desalting time are highly dependent on the sample quantity and
purity. They can be adjusted to meet customer needs. However, the desalting step must
be kept long enough to avoid the formation of adducts between salt and sample.
• Optimal trapping in the second dimension is achieved by adding a strong ion-pairing
agent to the fractions, prior to reinjection.
To Next Page
Loading...
