UltiMate 3000 RSLCnano – Standard Applications
47
2.12.2 Fluidic Setup
Figure 31 presents the setup using the parts of the Tandem nano LC application kit. Columns
are marked with letters, tubing with digits.
Tip: The schematic shows 10-port switching valves, but this application can be
performed on 6-port valves. Ensure that the relative positions of the connections
are correct, and update the valve switching in the Chromeleon templates if
necessary.
Figure 31: Fluidic connections for a Tandem nano LC experiment
A field upgrade to the WPS-3000FC needs to be performed to make the autosampler suitable
for this application. First, the nano injection kit (P/N 6824.0030) should be installed and
secondly the lower valve must be replaced with the nano switching valve (P/N 6825.0020).
All necessary parts are included in the kit. For further information, refer to the operating
instructions for the module.
Table 10: UltiMate 3000 RSLCnano Tandem nano LC kit (P/N 6720.0335) contents
#
Item Replacement P/N
a
75 µm I.D. x 15 cm, packed with Acclaim PepMap RSLC C18, 2 µm, 100Å,
nanoViper
164534
b
Nano Trap Column, 75 µm I.D. x 2 cm, packed with Acclaim PepMap100 C18,
3 µm, 100Å (set of 2) nanoViper
164535
1
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 20 µm x 350 mm 6041.5240
2,3
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 75 µm x 650 mm 6041.5775
4
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 75 µm x 250 mm 6041.5730
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 20 µm x 750 mm 6041.5280
5
PTFE tubing, 500 µm I.D. 100 cm, used as waste tubing 6720.0077
nanoViper sample loop 20 µL, FS/PEEK sheathed I.D. x L 250 µm x 408 mm 6826.2420
Fused silica tubing I.D. 20µm O.D. 280µm, 5 meters for nano LC connections 160475
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