Dionex UltiMate 3000 - Fluidic Setup

UltiMate 3000 RSLCnano – Standard Applications
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2.10.2 Fluidic Setup
Figure 27 presents the setup using the parts of the Automated Off-line Mic Nan SCX RP
application kit. Columns are marked with letters, tubing with digits.
Tip: The schematic shows 10-port switching valves, but this application can be
performed on 6-port valves. Ensure that the relative positions of the connections
are correct, and update the valve switching in the Chromeleon templates if
necessary.
Figure 27: Fluidic connections for an Automated Off-line 2D experiment
Table 8: UltiMate 3000 RSLCnano Automated Off-line SCX-RP peptides kit (P/N 6720.0330) contents
# Item Replacement P/N
a
1.0 mm I.D. x 15 cm, packed with Polysulfoethyl ASP, 5 µm, 300Å, nanoViper 164566
b
75 µm I.D. x 15 cm, packed with Acclaim PepMap RSLC C18, 2 µm, 100Å,
nanoViper
164534
c
Nano Trap column, 75 µm I.D. x 2 cm, packed with Acclaim PepMap100 C18,
3 µm, 100Å (set of 2) nanoViper
164535
1
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 20 µm x 350 mm 6041.5240
2
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 75 µm x 650 mm 6041.5775
3 (4)
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 75 µm x 550 mm 6041.5760
5
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 75 µm x 250 mm 6041.5730