Dionex UltiMate 3000 - Fluidic Setup

UltiMate 3000 RSLCnano – Standard Applications
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2.11.2 Fluidic Setup
Figure 29 presents the setup using the parts of the Automated Off-line Cap Nan application
kit. Columns are marked with letters, tubing with digits.
Tip: The schematic shows 10-port switching valves, but this application can be
performed on 6-port valves. Ensure that the relative positions of the connections
are correct, and update the valve switching in the Chromeleon templates if
necessary.
Figure 29: Fluidic connections for an Automated Off-line 2D experiment
Table 9: UltiMate 3000 RSLCnano Automated Off-line RP-RP peptides kit (P/N 6720.340) contents
# Item Replacement P/N
a
75 µm I.D. x 15 cm, packed with Acclaim PepMap RSLC C18, 2 µm, 100Å,
nanoViper
164534
b
Nano Trap Column, 75 µm I.D. x 2 cm, packed with Acclaim PepMap100 C18,
3 µm, 100Å (set of 2) nanoViper
164535
c
0.3 mm I.D. x 15 cm, packed with Acclaim PA2, nanoViper 164592
1
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 20 µm x 350 mm 6041.5240
2
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 50 µm x 350 mm 6041.5540
3
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 50 µm x 650 mm 6041.5575
4
nanoViper outlet tubing 50 um I.D. x 30 cm 6041.4573
Nano connector including connection sleeves 6720.0390
5
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 75 µm x 350 mm 6041.5735
6
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 75 µm x 250 mm 6041.5730
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 20 µm x 750 mm 6041.5280
nanoViper sample loop 20 µL, FS/PEEK sheathed I.D. x L 250 µm x 408 mm 6826.2420