UltiMate 3000 RSLCnano – Standard Applications
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2.10 Automated off-line 2D LC of Peptides, micro SCX x nano RP
2.10.1 Hardware Layout
Figure 25: Setup for an Automated Off-line 2D Mic Nan
experiment
The recommended setup with one
detector is presented in Figure 25,
when using two detectors a dual
stack, as shown in Figure 26, is
recommended. The single detector
setup consists of:
SRD-3400 5035.9245
NCS-3500RS 5041.0010
2x 10-port sw.valve 6041.0001
VWD-3400RS 5074.0010
3 nL flow cell 6074.0270
or
180 nL flow cell 6074.0290
WPS-3000TPL RS 5820.0010
µFC option 6820.0051
Application kit: 6720.0330
Tip: The µFC option limits
the upper pressure of the
first dimension to
350 bar, due to the
applied 8-port valve.
A second UV detector is
used when the nano
column is not directly
interfaced with the mass
spectrometer.
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