UltiMate 3000 RSLCnano – Standard Applications
6
2 Application Setup
2.1 General Recommendations for Applications
The experimental conditions for each application are presented together with related
information such as schematics, installation tips and examples, results and interpretation.
2.1.1 Connections
All high-pressure fittings used in the applications on the UltiMate 3000 RSLCnano system are
made with nanoViper. nanoViper is a fingertight high-pressure fitting that is dead volume free
by design and back pressure resistant up to 1000 bar. The fittings are assembled in the factory
to ensure consistent fittings and prevent experimental failure due to bad connections.
Figure 1: Example of a nanoViper fitting
1. Install nanoViper as any fingertight fitting.
2. Do not over-tighten connections (general guide line: finger-tight + maximum an
additional one eighth-turn).
3. Remove the black knurled screw.
The outlet of the nano columns is fitted with a nano connector. This is a dedicated connection
designed to offer maximum flexibility in connecting fused silica capillaries and offering
pressure stability up to 300 bars. The nano connector uses a special sleeve to ensure pressure
tightness. The assembly of a nano connector is described step by step below.
Figure 2: nano connector layout
• Use a new nano connector sleeve (P/N 6720.0391) for each connection
Important: Do NOT use a PTFE
sleeve. The size does not match the nano
connector and the pressure resistance is much lower.
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