UltiMate 3000 RSLCnano – Standard Applications
33
2.9.2 Fluidic Setup
Figure 24 presents the setup using the parts of the 2D-LC Salt Plugs application kit. Columns
are marked with letters, tubing with digits, and the sample loop is installed in the
WPS-3000PL Autosampler.
Tip: The schematic shows 10-port switching valves, but this application can be
performed on 6-port valves. Ensure that the relative positions of the connections
are correct, and update the valve switching in the Chromeleon templates if
necessary.
Figure 24: Fluidic connections for a 2D LC Salt Plugs experiment
Table 7: UltiMate 3000 RSLCnano 2D salt plugs kit (P/N 6720.0325) contents
# Item Replacement P/N
a
300 µm I.D. x 10 cm, packed with Poros 10 S with connections, 130 µm I.D.
FS sheathed inlet and outlet, nanoViper
164565
b
75 µm I.D. x 15 cm, packed with Acclaim PepMap RSLC C18, 2 µm, 100Å,
nanoViper
164534
c
Nano Trap Column, 100 µm I.D. x 2 cm, packed with Acclaim PepMap100 C18,
5 µm, 100Å (set of 2) nanoViper
164564
1
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 20 µm x 350 mm 6041.5240
2
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 75 µm x 650 mm 6041.5775
3
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 75 µm x 550 mm 6041.5760
4
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 75 µm x 250 mm 6041.5730
nanoViper capillary FS/PEEK sheathed 1/32" I.D. x L 20 µm x 750 mm 6041.5280
nanoViper sample loop 20 µL, FS/PEEK sheathed I.D. x L 250 µm x 408 mm 6826.2420
5
PTFE tubing, 500 µm I.D. 100 cm, used as waste tubing 6720.0077
Polypropylene vials for WPS with glass insert, 250 µL, 25 pcs. 6820.0027
Polypropylene caps for WPS vials, 25 pcs. 6820.0028
Protein mixture digest, 100 pmol, Lyophilized 161088
1/16" Universal Fingertight Fitting, one-piece design, extra long thread, 4 pcs. 6720.0015
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