Dionex UltiMate 3000 - Comprehensive 2 D LC

UltiMate 3000 RSLCnano – Standard Applications
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2.9.5 Salt Solutions Preparation
The following protocol can be used to prepare the salt plugs:
• Prepare two stock solutions using the loading solvent:
1) 2000 mM NaCl (for example, 467.5 mg of NaCl in 4 ml of loading solvent)
2) 100 mM NaCl (for example, prepare the 100 mM solution of the first table
two times)
• Dilute the stock according to the tables below: Use standard 1.5 mL vials (for example,
do not use inserts).
Concentration of
NaCl
Volume of 2000 mM NaCl
stock solution
Volume of loading
solvent
Total volume
2000 mM 1000 μL 0 μL 1000 μL
1000 mM 500 μL 500 μL 1000 µL
500 mM 250 μL 750 μL 1000 µL
200 mM 100 μL 900 μL 1000 µL
100 mM
50 μL 950 μL 1000 µL
Concentration of
NaCl
Volume of 100 mM NaCl
stock solution
Volume of
loading solvent
Total volume
50 mM 500 μL 500 μL 1000 µL
20 mM 200 μL 800μL 1000 µL
10 mM 100 μL 900 μL 1000 µL
5 mM 50 μL 950 μL 1000 µL
2 mM 20 μL 980 μL 1000 µL
1 mM 10 μL 990 μL 1000 µL