UltiMate 3000 RSLCnano – Standard Applications
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3.4.2 Unstable Baseline
Unstable baselines can have various causes. The UltiMate 3000 RSLCnano pumps are
designed to provide the best gradient precision, but solvent miscibility can present a problem.
Therefore, Thermo Fisher Scientific recommends using a minimum of 5% water in the
organic mobile phase.
Baseline artifacts in pre-concentration applications using low loading flows (< 10 µL/min)
may occur. These artifacts are only observed in the UV signal and have no effect on the
performance of the analysis. If artifacts in the baseline are observed, Thermo Fisher Scientific
recommends bypassing the degasser in pre-concentration applications where no gradient
formation is required and loading flows are below 20 µL/min. If bypassing the degasser is
undesired or impossible an alternative is to maintain degassing, but to increase the loading
flow during the elution phase to values between 30 and 100 µL/min.
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