UltiMate 3000 RSLCnano – Standard Applications
3
2.11.3 Installation tips ................................................................................................. 44
2.11.4 Testing the application ..................................................................................... 44
2.12 Tandem nano LC ..................................................................................................... 46
2.12.1 Hardware Layout .............................................................................................. 46
2.12.2 Fluidic Setup ..................................................................................................... 47
2.12.3 Installation Tips ................................................................................................ 48
2.12.4 Testing the Application .................................................................................... 48
3 FAQ ........................................................................................................................... 50
3.1 Interpreting a Chromatogram .................................................................................. 50
3.2 Troubleshooting nano LC peptide Applications...................................................... 50
3.3 The use of TFA and FA ........................................................................................... 52
3.4 Minimizing Baseline Noise ..................................................................................... 52
3.4.1 Drift .................................................................................................................. 52
3.4.2 Unstable Baseline ............................................................................................. 53
4 Appendix – Traditional Capillary Connections .................................................... 54
5 Appendix – Common Spare Parts in nano LC...................................................... 56
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