UltiMate 3000 RSLCnano – Standard Applications
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To evaluate the result of the experiment, the chromatogram should look very similar to the
one obtained with direct injection.
For details on interpretation and troubleshooting of the Cytochrome C nano LC separation,
see the FAQ section on page 50.
Tip: The example chromatogram provided (Figure 17) has been obtained under optimal
conditions. When having difficulties to obtain a similar result, focus on the delay of
the 'injection peak' and 'wash peak'; for example, check for any dwell (dead)
volume.
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